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control ad-green fluorescent protein (gfp) (ad-gfp  (Genechem)

 
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    Structured Review

    Genechem control ad-green fluorescent protein (gfp) (ad-gfp
    Control Ad Green Fluorescent Protein (Gfp) (Ad Gfp, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+ad-green+fluorescent+protein+(gfp)+(ad-gfp/control+ad+green+fluorescent+protein++gfp+++ad+gfp/pm36092165-72-8-15
    Average 90 stars, based on 1 article reviews
    control ad-green fluorescent protein (gfp) (ad-gfp - by Bioz Stars, 2026-10
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    Related Articles

    Control:

    Article Title: Mas‐Related G Protein‐Coupled Receptor Member D Sustains Hypertension
    Article Snippet: .. Ad‐MrgD and a control adenovirus expressing green fluorescent protein (Ad‐GFP) were obtained from Genechem (Shanghai, China). ..

    Expressing:

    Article Title: Mas‐Related G Protein‐Coupled Receptor Member D Sustains Hypertension
    Article Snippet: .. Ad‐MrgD and a control adenovirus expressing green fluorescent protein (Ad‐GFP) were obtained from Genechem (Shanghai, China). ..

    other:

    Article Title: RNF114 Interacts with EWSR1 to Regulate VEGFR2 in HER2-positive Breast Cancer
    Article Snippet: Ad-mCherry-GFP-LC3B vectors (Shanghai Genechem Co., Ltd.) were transfected into RNF114 knockdown and control SKBR3 cells.

    Article Title: MicroRNA-451a is a candidate biomarker and therapeutic target for major depressive disorder
    Article Snippet: For overexpression of miR-451a in vivo , viral constructs were designed using adeno-associated virus serotype 9 (AAV9) vectors (Genechem, China), encoding a pri-mmu-miR-451a-GFP fusion protein (Ad_OE-miR-451a) or green fluorescent protein (GFP) only as a negative control, under the control of the cytomegalovirus (CMV) promoter (Ad_OE-scramble).

    Article Title: Empagliflozin ameliorates diabetic cardiomyopathy probably via activating AMPK/PGC-1α and inhibiting the RhoA/ROCK pathway
    Article Snippet: Next, the cells were cultured in different conditions: 5.5 mmol/L D-glucose as normal control (NG) group; 5.5 mmol/L D-glucose plus 24.5 mmol/L D-mannitol as hyperosmotic group (OSM); 30 mmol/L D-glucose as HG group (HG); cells in the HG + EM group were pretreated with 10 μM empagliflozin for 2 h and then incubated with HG for an additional 48 h; cells in the HG + EM + compound C (CC) group were pretreated with AMPK inhibitor and 1 μM CC (MCE, CA, United States) for 2 h, and then the cells were cultured as in the HG + EM group; cells in the HG + FA group were pretreated with 100 μM FA (Hongri, Tianjin, China)) as our previous study[ ] and incubated with HG for an additional 48 h; cells in the PGC-1α-GFP-Ad and GFP-Ad groups were transfected with PGC-1α-GFP-Ad and GFP-Ad (Shanghai Genechem Co., Ltd., China), respectively, using liposomes (Invitrogen, United States) and then incubated with HG for an additional 48 h.

    Article Title: The deubiquitinase OTUD1 stabilizes NRF2 to alleviate hepatic ischemia/reperfusion injury.
    Article Snippet: Hepatic ischemia/reperfusion (I/R) injury is an important cause of liver function impairment following liver surgery.. The ubiquitin-proteasome system (UPS) plays a crucial role in protein quality control and has substantial impact on the hepatic I/R process.. Although OTU deubiquitinase 1 (OTUD1) is involved in diverse biological processes, its specific functional implications in hepatic I/R are not yet fully understood.

    Article Title: Protein S-glutathionylation confers cell resistance to ferroptosis
    Article Snippet: The CHAC1 (Ad-CHAC1) and control (Ad-GFP) adenoviruses were purchased from GeneChem Technologies Co., Ltd. (Shanghai, China).

    Recombinant:

    Article Title: CD9 promotes TβR2–TβR1 association driving the transition of human dermal fibroblasts to myofibroblast under hypoxia
    Article Snippet: The α-SMA and CD9 expression were observed under Leica Confocal Microscope (Leica Microsystems, Wetzlar, Germany). .. The recombinant adenovirus vectors for CD9 overexpressing (Ad-CD9-GFP) and the negative control adenovirus vectors encoded the GFP sequence (vector) were purchased from Shanghai GeneChem, Co. Ltd (Shanghai, China). ..

    Negative Control:

    Article Title: CD9 promotes TβR2–TβR1 association driving the transition of human dermal fibroblasts to myofibroblast under hypoxia
    Article Snippet: The α-SMA and CD9 expression were observed under Leica Confocal Microscope (Leica Microsystems, Wetzlar, Germany). .. The recombinant adenovirus vectors for CD9 overexpressing (Ad-CD9-GFP) and the negative control adenovirus vectors encoded the GFP sequence (vector) were purchased from Shanghai GeneChem, Co. Ltd (Shanghai, China). ..

    Sequencing:

    Article Title: CD9 promotes TβR2–TβR1 association driving the transition of human dermal fibroblasts to myofibroblast under hypoxia
    Article Snippet: The α-SMA and CD9 expression were observed under Leica Confocal Microscope (Leica Microsystems, Wetzlar, Germany). .. The recombinant adenovirus vectors for CD9 overexpressing (Ad-CD9-GFP) and the negative control adenovirus vectors encoded the GFP sequence (vector) were purchased from Shanghai GeneChem, Co. Ltd (Shanghai, China). ..



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    HuR modulated endothelial apoptosis and dysfunction. (A) The levels of HuR protein and mRNA were measured in EOMA cells treated with H 2 O 2. (B) The luciferase activity was analyzed in EOMA cells transfected with luciferase reporter vector containing the HuR coding region or 3′-UTR. The protein level of HUR was analyzed in EOMA cells transfected with (C) 291m or (D) 291i. (E) The mRNA level of HuR was analyzed by qPCR in EOMA cells transfected with 291m or 291i. (F) The levels of apoptosis in EOMA cells transfected AD-HUR or control <t>AD-CON</t> vectors was measured by TUNEL staining. (G) The mRNA levels of ICAM-1 and VCAM-1 in EOMA cells transfected with AD-HUR or AD-CON were measured by qPCR. (H) The levels of p-ERK, Bax and Bcl-2 were analyzed by western blot analysis. Data are presented as the mean ± standard error of the mean (n=5). * P<0.05, ** P<0.01 and *** P<0.001 vs. control. Con/NC, negative control; miRNA, microRNA; NCI, miRNA inhibitor negative control; qPCR, quantitative polymerase chain reaction; TUNEL, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labelling; ICAM-1, intercellular adhesion molecule-1; VCAM-1, vascular cell adhesion molecule-1; ERK, extracellular signal-regulated kinase; p-ERK, phosphorylated ERK; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; HuR, Hu antigen R; AD-CON, recombinant adenovirus vector expressing mouse HuR; AD-HUR, adenovirus vector containing <t>green</t> <t>fluorescent</t> <t>protein;</t> H 2 O 2 , hydrogen peroxide; 291m, miR-291b-3p mimics; 291i, miR-291b-3p inhibitor; UTR, untranslated region.
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    HuR modulated endothelial apoptosis and dysfunction. (A) The levels of HuR protein and mRNA were measured in EOMA cells treated with H 2 O 2. (B) The luciferase activity was analyzed in EOMA cells transfected with luciferase reporter vector containing the HuR coding region or 3′-UTR. The protein level of HUR was analyzed in EOMA cells transfected with (C) 291m or (D) 291i. (E) The mRNA level of HuR was analyzed by qPCR in EOMA cells transfected with 291m or 291i. (F) The levels of apoptosis in EOMA cells transfected AD-HUR or control <t>AD-CON</t> vectors was measured by TUNEL staining. (G) The mRNA levels of ICAM-1 and VCAM-1 in EOMA cells transfected with AD-HUR or AD-CON were measured by qPCR. (H) The levels of p-ERK, Bax and Bcl-2 were analyzed by western blot analysis. Data are presented as the mean ± standard error of the mean (n=5). * P<0.05, ** P<0.01 and *** P<0.001 vs. control. Con/NC, negative control; miRNA, microRNA; NCI, miRNA inhibitor negative control; qPCR, quantitative polymerase chain reaction; TUNEL, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labelling; ICAM-1, intercellular adhesion molecule-1; VCAM-1, vascular cell adhesion molecule-1; ERK, extracellular signal-regulated kinase; p-ERK, phosphorylated ERK; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; HuR, Hu antigen R; AD-CON, recombinant adenovirus vector expressing mouse HuR; AD-HUR, adenovirus vector containing <t>green</t> <t>fluorescent</t> <t>protein;</t> H 2 O 2 , hydrogen peroxide; 291m, miR-291b-3p mimics; 291i, miR-291b-3p inhibitor; UTR, untranslated region.
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    Image Search Results


    HuR modulated endothelial apoptosis and dysfunction. (A) The levels of HuR protein and mRNA were measured in EOMA cells treated with H 2 O 2. (B) The luciferase activity was analyzed in EOMA cells transfected with luciferase reporter vector containing the HuR coding region or 3′-UTR. The protein level of HUR was analyzed in EOMA cells transfected with (C) 291m or (D) 291i. (E) The mRNA level of HuR was analyzed by qPCR in EOMA cells transfected with 291m or 291i. (F) The levels of apoptosis in EOMA cells transfected AD-HUR or control AD-CON vectors was measured by TUNEL staining. (G) The mRNA levels of ICAM-1 and VCAM-1 in EOMA cells transfected with AD-HUR or AD-CON were measured by qPCR. (H) The levels of p-ERK, Bax and Bcl-2 were analyzed by western blot analysis. Data are presented as the mean ± standard error of the mean (n=5). * P<0.05, ** P<0.01 and *** P<0.001 vs. control. Con/NC, negative control; miRNA, microRNA; NCI, miRNA inhibitor negative control; qPCR, quantitative polymerase chain reaction; TUNEL, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labelling; ICAM-1, intercellular adhesion molecule-1; VCAM-1, vascular cell adhesion molecule-1; ERK, extracellular signal-regulated kinase; p-ERK, phosphorylated ERK; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; HuR, Hu antigen R; AD-CON, recombinant adenovirus vector expressing mouse HuR; AD-HUR, adenovirus vector containing green fluorescent protein; H 2 O 2 , hydrogen peroxide; 291m, miR-291b-3p mimics; 291i, miR-291b-3p inhibitor; UTR, untranslated region.

    Journal: International Journal of Molecular Medicine

    Article Title: miR-291b-3p mediated ROS-induced endothelial cell dysfunction by targeting HUR

    doi: 10.3892/ijmm.2018.3821

    Figure Lengend Snippet: HuR modulated endothelial apoptosis and dysfunction. (A) The levels of HuR protein and mRNA were measured in EOMA cells treated with H 2 O 2. (B) The luciferase activity was analyzed in EOMA cells transfected with luciferase reporter vector containing the HuR coding region or 3′-UTR. The protein level of HUR was analyzed in EOMA cells transfected with (C) 291m or (D) 291i. (E) The mRNA level of HuR was analyzed by qPCR in EOMA cells transfected with 291m or 291i. (F) The levels of apoptosis in EOMA cells transfected AD-HUR or control AD-CON vectors was measured by TUNEL staining. (G) The mRNA levels of ICAM-1 and VCAM-1 in EOMA cells transfected with AD-HUR or AD-CON were measured by qPCR. (H) The levels of p-ERK, Bax and Bcl-2 were analyzed by western blot analysis. Data are presented as the mean ± standard error of the mean (n=5). * P<0.05, ** P<0.01 and *** P<0.001 vs. control. Con/NC, negative control; miRNA, microRNA; NCI, miRNA inhibitor negative control; qPCR, quantitative polymerase chain reaction; TUNEL, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labelling; ICAM-1, intercellular adhesion molecule-1; VCAM-1, vascular cell adhesion molecule-1; ERK, extracellular signal-regulated kinase; p-ERK, phosphorylated ERK; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; HuR, Hu antigen R; AD-CON, recombinant adenovirus vector expressing mouse HuR; AD-HUR, adenovirus vector containing green fluorescent protein; H 2 O 2 , hydrogen peroxide; 291m, miR-291b-3p mimics; 291i, miR-291b-3p inhibitor; UTR, untranslated region.

    Article Snippet: Recombinant adenovirus vectors expressing mouse HuR (AD-HUR) and control adenovirus vectors containing green fluorescent protein (GFP) (AD-CON) were purchased from Shanghai GeneChem Co., Ltd. (Shanghai, China).

    Techniques: Luciferase, Activity Assay, Transfection, Plasmid Preparation, TUNEL Assay, Staining, Western Blot, Negative Control, Real-time Polymerase Chain Reaction, Recombinant, Expressing

    miR-291b-3p regulates apoptosis and dysfunction of EOMA cells via targeting HuR. (A) The levels of apoptosis in EOMA cells co-transfected with miR-291b-3p mimics and AD-HUR was measured by TUNEL staining. (B) The mRNA levels of ICAM-1 and VCAM-1 were measured by quantitative polymerase chain reaction. (C) The levels of p-ERK, Bax and Bcl-2 were analyzed by western blot analysis. Data are presented as the mean ± standard error of the mean (n=5). * P<0.05, ** P<0.01 and *** P<0.001 vs. control. miR, microRNA; NC, negative control; TUNEL, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labelling; ICAM-1, intercellular adhesion molecule-1; VCAM-1, vascular cell adhesion molecule-1; ERK, extracellular signal-regulated kinase; p-ERK, phosphorylated ERK; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; NCI, miRNA inhibitor negative control; 291m, miR-291b-3p mimic; 291i, miR-291b-3p inhibitor; HuR, Hu antigen R; AD-CON, recombinant adenovirus vector expressing mouse HuR; AD-HUR, adenovirus vector containing green fluorescent protein.

    Journal: International Journal of Molecular Medicine

    Article Title: miR-291b-3p mediated ROS-induced endothelial cell dysfunction by targeting HUR

    doi: 10.3892/ijmm.2018.3821

    Figure Lengend Snippet: miR-291b-3p regulates apoptosis and dysfunction of EOMA cells via targeting HuR. (A) The levels of apoptosis in EOMA cells co-transfected with miR-291b-3p mimics and AD-HUR was measured by TUNEL staining. (B) The mRNA levels of ICAM-1 and VCAM-1 were measured by quantitative polymerase chain reaction. (C) The levels of p-ERK, Bax and Bcl-2 were analyzed by western blot analysis. Data are presented as the mean ± standard error of the mean (n=5). * P<0.05, ** P<0.01 and *** P<0.001 vs. control. miR, microRNA; NC, negative control; TUNEL, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labelling; ICAM-1, intercellular adhesion molecule-1; VCAM-1, vascular cell adhesion molecule-1; ERK, extracellular signal-regulated kinase; p-ERK, phosphorylated ERK; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; NCI, miRNA inhibitor negative control; 291m, miR-291b-3p mimic; 291i, miR-291b-3p inhibitor; HuR, Hu antigen R; AD-CON, recombinant adenovirus vector expressing mouse HuR; AD-HUR, adenovirus vector containing green fluorescent protein.

    Article Snippet: Recombinant adenovirus vectors expressing mouse HuR (AD-HUR) and control adenovirus vectors containing green fluorescent protein (GFP) (AD-CON) were purchased from Shanghai GeneChem Co., Ltd. (Shanghai, China).

    Techniques: Transfection, TUNEL Assay, Staining, Real-time Polymerase Chain Reaction, Western Blot, Negative Control, Recombinant, Plasmid Preparation, Expressing